human il-1β elisa kit  (Multi Sciences (Lianke) Biotech Co Ltd)

Journal: Cell Reports Medicine

Article Title: A biomimetic hybrid membrane vesicle nanoplatform attenuates tendinopathy through neuroinflammation modulation and tendon regeneration

doi: 10.1016/j.xcrm.2026.102937

Figure Lengend Snippet: MSM-DTM adsorbs inflammatory cytokines, suppresses TSPCs inflammation and senescence, and promotes tenogenic differentiation and migration (A) The remaining concentrations of TNF-α, IL-1β, IL-6, IFN-γ, LPS, and NGF were detected by ELISA after co-culture with different concentrations of MSM. (B and C) RT-qPCR of IL-6 and CCL-2 in TSPCs under different treatments. (D) Western blot of COL1A2, MMP3, and TNMD. (E) ELISA of TNF-α, IL-6, and IFN-γ in supernatants. (F)Western blot of p16 and p21 after 7 days of treatment. (G) β-Galactosidase staining analysis after 7 days of treatment. Scale bars, 100 μm. (H and I) Immunofluorescence and quantification of TNMD and TNC after 14-day treatment. Scale bars, 50 μm. (J) Relative mRNA expression of tenogenic differentiation markers SCX and TNC. (K and L) Representative images and quantification of Transwell assay. Scale bars, 100 μm. (M and N) Representative images and quantification of wound healing assay. Scale bars, 100 μm. Statistical comparisons were performed with one-way ANOVA with Tukey’s multiple comparisons test. Data are presented as the mean ± SD, n = 3. Ns, no significance, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 between groups.

Article Snippet: IL-1β ELISA kit , ABclonal , RK00001.

Techniques: Migration, Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Quantitative RT-PCR, Western Blot, Staining, Immunofluorescence, Expressing, Transwell Assay, Wound Healing Assay

Journal: Cell Reports Medicine

Article Title: A biomimetic hybrid membrane vesicle nanoplatform attenuates tendinopathy through neuroinflammation modulation and tendon regeneration

doi: 10.1016/j.xcrm.2026.102937

Figure Lengend Snippet: MSM-DTM alleviates TSPCs senescence via JAK-STAT pathway suppression (A) Volcano plot of differentially expressed genes between control and IL-1β groups. (B) Heatmap of gene expression in control, IL-1β, and MSM-DTM groups. (C) KEGG pathway analysis. (D) Excessive activation of the JAK-STAT pathway induces senescence of TSPCs. (E) Western blot of p-JAK2, JAK2, p-STAT3, and STAT3 in TSPCs. (F) Western blot of p16 and p21 in TSPCs. (G) β-Galactosidase staining analysis after 7 days of treatment. Scale bars, 100 μm. (H) The ratio of phosphorylated STAT3 expression to total STAT3 expression. (I and J) Relative expression levels of p16 and p21 proteins. Statistical comparisons were performed with one-way ANOVA with Tukey’s multiple comparisons test. Data are presented as the mean ± SD, n = 3. Ns, no significance, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 between groups. BP, biological process; CC, cellular component; MF, molecular function.

Article Snippet: IL-1β ELISA kit , ABclonal , RK00001.

Techniques: Control, Gene Expression, Activation Assay, Western Blot, Staining, Expressing

Journal: Cell Reports Medicine

Article Title: A biomimetic hybrid membrane vesicle nanoplatform attenuates tendinopathy through neuroinflammation modulation and tendon regeneration

doi: 10.1016/j.xcrm.2026.102937

Figure Lengend Snippet:

Article Snippet: IL-1β ELISA kit , ABclonal , RK00001.

Techniques: Recombinant, Lysis, Hydroxyproline Assay, Enzyme-linked Immunosorbent Assay, Isolation, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Staining, Software

Journal: American Journal of Translational Research

Article Title: AGTR1 inhibits pyroptosis and inflammatory cytokine secretion in rheumatoid arthritis fibroblast-like synoviocytes through the TGF-β pathway

doi: 10.62347/HTCE3264

Figure Lengend Snippet: AGTR1 regulates pyroptosis and inflammatory cytokine secretion. A, B. TUNEL staining of FLS treated with oe-NC, oe-NC+Nigericin, or oe-AGTR1+Nigericin. C, D. Flow cytometry analysis of caspase-1-positive cells. E-H. ELISA measurement of IL-1β, IL-18, IL-6, and IL-8 levels in culture supernatants. I. LDH release assay of FLS with different treatments. J. Representative images of dual immunofluorescence staining for PI (red) and Hoechst 33342 (blue).

Article Snippet: Secretion of IL-8 (E-EL-H6008), IL-18 (E-EL-H0253), IL-1β (E-EL-H0149), and IL-6 (E-EL-H6156) in RA-FLS supernatants was quantified using ELISA kits (Elabscience, China).

Techniques: TUNEL Assay, Staining, Flow Cytometry, Analysis, Enzyme-linked Immunosorbent Assay, Lactate Dehydrogenase Assay, Immunofluorescence

Journal: American Journal of Translational Research

Article Title: AGTR1 inhibits pyroptosis and inflammatory cytokine secretion in rheumatoid arthritis fibroblast-like synoviocytes through the TGF-β pathway

doi: 10.62347/HTCE3264

Figure Lengend Snippet: AGTR1 inhibits FLS pyroptosis and inflammatory cytokine secretion through the TGF-β pathway. A, B. WB detection of P-SMAD2, SMAD2, and TGF-β expression in FLS cells treated with oe-NC, oe-NC+Nigericin, and oe-AGTR1+Nigericin. C. The ratio of P-SMAD2 to total SMAD2 in FLS cells. D-F. WB detection of ASC, NLRP3, cleaved caspase-1, and GSDMD-N protein expression levels in RA-FLS cells under Nigericin induction after treatment with oe-NC, oe-AGTR1, and oe-AGTR1+SB505124 (TGF-β receptor I inhibitor). G, H. TUNEL staining to assess apoptosis in FLS cells after different treatments. I, J. Flow cytometry detection of caspase-1-positive FLS cells after different treatments. K-N. ELISA measurement of inflammatory cytokines IL-1β, IL-18, IL-6, and IL-8 levels in the supernatant of FLS cells after different treatments. O. LDH release assay of FLS with different treatments. P. Representative images of dual fluorescence staining with PI (red) and Hoechst 33342 (blue).

Article Snippet: Secretion of IL-8 (E-EL-H6008), IL-18 (E-EL-H0253), IL-1β (E-EL-H0149), and IL-6 (E-EL-H6156) in RA-FLS supernatants was quantified using ELISA kits (Elabscience, China).

Techniques: Expressing, TUNEL Assay, Staining, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Lactate Dehydrogenase Assay, Fluorescence

Journal: International Journal of Molecular Sciences

Article Title: Mogroside V Alleviates Renal Injury in Diabetic Mice via Regulation of the TLR4 / NF-κB Pathway and Modulation of ECM Remodeling

doi: 10.3390/ijms27146271

Figure Lengend Snippet: MV suppressed inflammatory responses. ( A ) The levels of IL-6, IL-18, IL-1β, TNF-α and MCP-1 in the supernatant were detected using ELISA after treatment with MV (25, 50, 100 mg/kg/d) or Met (101.4 mg/kg/d) in the serum of db/db mice (n = 3). ( B ) The levels of TNF-α, IL-1β, MCP-1 and IL-18 in the supernatant were detected using ELISA after treatment with HG in the presence or absence of MV or Met for 48 h in HRMC. ** p < 0.01 vs. respective control group; # p < 0.05 vs. respective DKD group (DKD mice for panel A, HG-treated HRMC for panel B); ## p < 0.01 vs. respective DKD group.

Article Snippet: ELISA kits for TNF-α (Cat: EK182), IL-1β (Cat: EK101B), IL-6 (Cat: EK106), MCP-1 (Cat: EK187), and IL-18 (Cat: EK118) were purchased from Hangzhou Lianke Biotech Co., Ltd. (Hangzhou, China).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Mogroside V Alleviates Renal Injury in Diabetic Mice via Regulation of the TLR4 / NF-κB Pathway and Modulation of ECM Remodeling

doi: 10.3390/ijms27146271

Figure Lengend Snippet: Effect of MV on inflammatory factors. ( A ) Western blot analysis was used to detect the expression of inflammatory proteins TNF-α, IL-1β, and IL-18 in db/db mice. ( B ) Western blot analysis was used to detect the expression of inflammatory proteins TNF-α, IL-1β, MCP-1 and IL-6 in HRMC. ** p < 0.01 vs. respective control group; # p < 0.05 vs. respective DKD group (DKD mice for panel A, HG-treated HRMC for panel B); ## p < 0.01 vs. respective DKD group.

Article Snippet: ELISA kits for TNF-α (Cat: EK182), IL-1β (Cat: EK101B), IL-6 (Cat: EK106), MCP-1 (Cat: EK187), and IL-18 (Cat: EK118) were purchased from Hangzhou Lianke Biotech Co., Ltd. (Hangzhou, China).

Techniques: Western Blot, Expressing, Control